I’ve just done a full backup of the root folder and 256 databases before doing the WordPressMu upgrade to 2.8.5.2
Everything seemed to go quite smoothly, I had to update the multidb file db.php in wp-content to 2.8.5 due to the new way WordPress upgrades the database but apart from that it was simple.
This is why I love having a backup of the server running on my one at home. I can practice all these things on the latest backup and see what happens in a controlled environment first. If you notice any problems accessing your blog let me know through the support ticket system.